Set ptimer melting temperatures
primerTm(settings, min = NULL, optimal = NULL, max = NULL)
| settings | a settings list created by `p3Settings` |
|---|---|
| min | minimal temperature (PRIMER_MAX_TM) |
| optimal | optimal temperature (PRIMER_OPT_TM) |
| max | maximal temperature (PRIMER_MIN_TM) |
a settings list with updated values
#> List of 115 #> $ P3_FILE_TYPE : chr "settings" #> $ P3_FILE_ID : chr "Default settings of primer3 version 1.1.4" #> $ PRIMER_TASK : chr "pick_detection_primers" #> $ PRIMER_PICK_LEFT_PRIMER : chr "1" #> $ PRIMER_PICK_INTERNAL_OLIGO : chr "0" #> $ PRIMER_PICK_RIGHT_PRIMER : chr "1" #> $ PRIMER_NUM_RETURN : chr "5" #> $ PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION: chr "5" #> $ PRIMER_PRODUCT_SIZE_RANGE : chr "100-300" #> $ PRIMER_PRODUCT_OPT_SIZE : chr "0" #> $ PRIMER_PAIR_WT_PRODUCT_SIZE_LT : chr "0.0" #> $ PRIMER_PAIR_WT_PRODUCT_SIZE_GT : chr "0.0" #> $ PRIMER_MIN_SIZE : chr "18" #> $ PRIMER_INTERNAL_MIN_SIZE : chr "18" #> $ PRIMER_OPT_SIZE : chr "20" #> $ PRIMER_INTERNAL_OPT_SIZE : chr "20" #> $ PRIMER_MAX_SIZE : chr "27" #> $ PRIMER_INTERNAL_MAX_SIZE : chr "27" #> $ PRIMER_WT_SIZE_LT : chr "1.0" #> $ PRIMER_INTERNAL_WT_SIZE_LT : chr "1.0" #> $ PRIMER_WT_SIZE_GT : chr "1.0" #> $ PRIMER_INTERNAL_WT_SIZE_GT : chr "1.0" #> $ PRIMER_MIN_GC : chr "20.0" #> $ PRIMER_INTERNAL_MIN_GC : chr "20.0" #> $ PRIMER_OPT_GC_PERCENT : chr "50.0" #> $ PRIMER_MAX_GC : chr "80.0" #> $ PRIMER_INTERNAL_MAX_GC : chr "80.0" #> $ PRIMER_WT_GC_PERCENT_LT : chr "0.0" #> $ PRIMER_INTERNAL_WT_GC_PERCENT_LT : chr "0.0" #> $ PRIMER_WT_GC_PERCENT_GT : chr "0.0" #> $ PRIMER_INTERNAL_WT_GC_PERCENT_GT : chr "0.0" #> $ PRIMER_GC_CLAMP : chr "0" #> $ PRIMER_MAX_END_GC : chr "5" #> $ PRIMER_MIN_TM : num 58 #> $ PRIMER_INTERNAL_MIN_TM : chr "57.0" #> $ PRIMER_OPT_TM : num 60 #> $ PRIMER_INTERNAL_OPT_TM : chr "60.0" #> $ PRIMER_MAX_TM : num 62 #> $ PRIMER_INTERNAL_MAX_TM : chr "63.0" #> $ PRIMER_PAIR_MAX_DIFF_TM : chr "100.0" #> $ PRIMER_WT_TM_LT : chr "1.0" #> $ PRIMER_INTERNAL_WT_TM_LT : chr "1.0" #> $ PRIMER_WT_TM_GT : chr "1.0" #> $ PRIMER_INTERNAL_WT_TM_GT : chr "1.0" #> $ PRIMER_PAIR_WT_DIFF_TM : chr "0.0" #> $ PRIMER_PRODUCT_MIN_TM : chr "-1000000.0" #> $ PRIMER_PRODUCT_OPT_TM : chr "0.0" #> $ PRIMER_PRODUCT_MAX_TM : chr "1000000.0" #> $ PRIMER_PAIR_WT_PRODUCT_TM_LT : chr "0.0" #> $ PRIMER_PAIR_WT_PRODUCT_TM_GT : chr "0.0" #> $ PRIMER_TM_FORMULA : chr "0" #> $ PRIMER_SALT_MONOVALENT : chr "50.0" #> $ PRIMER_INTERNAL_SALT_MONOVALENT : chr "50.0" #> $ PRIMER_SALT_DIVALENT : chr "0.0" #> $ PRIMER_INTERNAL_SALT_DIVALENT : chr "0.0" #> $ PRIMER_DNTP_CONC : chr "0.0" #> $ PRIMER_INTERNAL_DNTP_CONC : chr "0.0" #> $ PRIMER_SALT_CORRECTIONS : chr "0" #> $ PRIMER_DNA_CONC : chr "50.0" #> $ PRIMER_INTERNAL_DNA_CONC : chr "50.0" #> $ PRIMER_MAX_SELF_ANY : chr "8.00" #> $ PRIMER_INTERNAL_MAX_SELF_ANY : chr "12.00" #> $ PRIMER_PAIR_MAX_COMPL_ANY : chr "8.00" #> $ PRIMER_WT_SELF_ANY : chr "0.0" #> $ PRIMER_INTERNAL_WT_SELF_ANY : chr "0.0" #> $ PRIMER_PAIR_WT_COMPL_ANY : chr "0.0" #> $ PRIMER_MAX_SELF_END : chr "3.00" #> $ PRIMER_INTERNAL_MAX_SELF_END : chr "12.00" #> $ PRIMER_PAIR_MAX_COMPL_END : chr "3.00" #> $ PRIMER_WT_SELF_END : chr "0.0" #> $ PRIMER_INTERNAL_WT_SELF_END : chr "0.0" #> $ PRIMER_PAIR_WT_COMPL_END : chr "0.0" #> $ PRIMER_MAX_END_STABILITY : chr "100.0" #> $ PRIMER_WT_END_STABILITY : chr "0.0" #> $ PRIMER_MAX_NS_ACCEPTED : chr "0" #> $ PRIMER_INTERNAL_MAX_NS_ACCEPTED : chr "0" #> $ PRIMER_WT_NUM_NS : chr "0.0" #> $ PRIMER_INTERNAL_WT_NUM_NS : chr "0.0" #> $ PRIMER_MAX_POLY_X : chr "5" #> $ PRIMER_INTERNAL_MAX_POLY_X : chr "5" #> $ PRIMER_MIN_THREE_PRIME_DISTANCE : chr "-1" #> $ PRIMER_PICK_ANYWAY : chr "0" #> $ PRIMER_LOWERCASE_MASKING : chr "0" #> $ PRIMER_EXPLAIN_FLAG : chr "0" #> $ PRIMER_LIBERAL_BASE : chr "0" #> $ PRIMER_FIRST_BASE_INDEX : chr "0" #> $ PRIMER_MAX_TEMPLATE_MISPRIMING : chr "-1.00" #> $ PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING : chr "-1.00" #> $ PRIMER_WT_TEMPLATE_MISPRIMING : chr "0.0" #> $ PRIMER_PAIR_WT_TEMPLATE_MISPRIMING : chr "0.0" #> $ PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS : chr "1" #> $ PRIMER_MAX_LIBRARY_MISPRIMING : chr "12.00" #> $ PRIMER_INTERNAL_MAX_LIBRARY_MISHYB : chr "12.00" #> $ PRIMER_PAIR_MAX_LIBRARY_MISPRIMING : chr "24.00" #> $ PRIMER_WT_LIBRARY_MISPRIMING : chr "0.0" #> $ PRIMER_INTERNAL_WT_LIBRARY_MISHYB : chr "0.0" #> $ PRIMER_PAIR_WT_LIBRARY_MISPRIMING : chr "0.0" #> $ PRIMER_MIN_QUALITY : chr "0" #> $ PRIMER_INTERNAL_MIN_QUALITY : chr "0" #> [list output truncated]