Estimate initial guess for the mean expression level

guessMeans(pulseData, totalLabel = "total", fun = c("mean", "geomean",
  "median"))

Arguments

pulseData

the PulseData object

totalLabel

a character, the name of the factor level in the condition matrix, which correspond to the total fraction ("total" by default).

fun

a function used to estimate the expression level. Possible variants are mean, median and adjusted geometric mean (i.e. $exp(mean(log(x + .5)))$.

Value

a vector of expression level estimations for every gene

Details

Use this function to estimate mean read numbers on the basis of the total fraction.