Estimate initial guess for the mean expression level
guessMeans(pulseData, totalLabel = "total", fun = c("mean", "geomean", "median"))
pulseData | the |
---|---|
totalLabel | a character, the name of the factor level in the condition matrix, which correspond to the total fraction ("total" by default). |
fun | a function used to estimate the expression level. Possible variants are mean, median and adjusted geometric mean (i.e. $exp(mean(log(x + .5)))$. |
a vector of expression level estimations for every gene
Use this function to estimate mean read numbers on the basis of the total fraction.